The first step in creating the St. Johns River-to-Sea Bike Loop wildflower trail in time for Florida’s quincentennial in 2013 is to determine existing and potential wildflower sites along the bike loop. While spring and fall are the two major seasons for showy wildflower displays in Florida, a summer survey was warranted in order to develop a more comprehensive evaluation of existing and potential wildflower sites. This report represents the results of our site evaluations based on our observations in May, July, and October 2009. It supersedes our May and July 2009 survey reports submitted to the Foundation.